Journal: BMC Complementary and Alternative Medicine
Article Title: Jia-Wei-Jiao-Tai-Wan ameliorates type 2 diabetes by improving β cell function and reducing insulin resistance in diabetic rats
doi: 10.1186/s12906-017-2016-5
Figure Lengend Snippet: Effect of JWJTW on insulin signaling in skeletal muscle of diabetic rats. Western blot analysis of phospho-InsR-β Tyr1361 ( a ), phospho-IRS-1 Tyr612 and phospho-IRS-1 Ser307 ( b ), PI3K (p85) ( c ) and GLUT4 ( d ). * p < 0.05, ** p < 0.01, *** p < 0.001 ( n = 5)
Article Snippet: The antibodies used in this study were purchased from the following manufacturers: the antibody against pancreatic and duodenal homeobox 1 (PDX-1) was purchased from Chemi-con (USA); against insulin and against insulin receptor (InsR-β) from Abcam (UK); against insulin receptor substrate (IRS)-1 from Upstate (USA); against phospho-insulin receptor substrate (pIRS)-1(Ser307) and pIRS-1(Tyr612), phospho-insulin receptor (pInsR)-β (Tyr1361), phosphatidylinositol 3-kinase (PI3K) (p85) and glucose transporter 4 (GLUT4) from Cell Signaling Technology (CST, USA) and against GAPDH from Wuhan Gugeshengwu Technology Co., Ltd. (China).
Techniques: Western Blot